Biology Notes Home Page

GENETIC TECHNOLOGY

I. Genomics
        Genome: 

 

        Haploid (n)
        Diploid (2n)
        Germ cell 
        Germ line

        Somatic cell

About 99%of our DNA are noncoding sequences.

II.  Cloning
Reproductive cloning:
 
Therapeutic cloning:
Nuclear transfer

nuclear transfer

source Campbell, Reece, Tyler, & Simons (2006) .Biology Concepts and Connection. Benjamin Cummings San Francisco

III. Stem Cells
    Stages of Development
1.Oocyte:

Ovum: 

Zygote:
Embryo: 
5.Fetus:
An unspecified cell that has the ability to divide for indefinite periods of time and give rise to a specialized cell.

 

EMBRYONIC
ADULT
Have the ability to:
Self-regenerate
Have the ability to:
Self-regenerate
differentiate into other cells all other cell types (called plueripotent)
differentiate into limited cell types.  (called unipotent)
embryonic stem cell embryonic stem cell
http://www.csa.com/discoveryguides/stemcell/overview.php

 

http://www.csa.com/discoveryguides/stemcell/images/differ.jpg

 

 

IV.  Genetic engineering

A.        A.  Transgenic 

Animals and plants in which novel DNA has been incorporated in the genetic line.

i.e. Golden rice

 

Frankenfood

 

B.  Recombinant DNA also called Gene Cloning

An enzyme breaks the DNA and a fragment of DNA is attached into the DNA strand.
 
The DNA is “pasted” together with enzymes.
 
Now the organism can produce the proteins on the new DNA strand.

C.  Genetic Therapy

gene vectors:

A piece of DNA usually a viral or bacterial that moves genes from one cell to another.

D. Genetic testing or screening

A sample of tissue is run through an electrophoresis unit, which physically sorts the nucleic acids.
 
A genetic marker is determined because DNA material varies from person to person

 

1. Disease

DNA testing can indicate the existence
of a particular genetic trait,
changes in chromosomes
changes in DNA that are associated with inherited diseases in asymptomatic individuals

2. DNA profiling also called DNA fingerprinting

Sample is then segmented using enzymes
 
the segments are arranged by size using a process called electrophoresis.
 
The segments are marked & exposed to X-ray where they form a pattern of black bars—
 
If the DNA fingerprints produced from two different samples match, the two samples probably came from the same person.